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In trimethylammonium acetate solutions and in soft resource problems, the protonated phosphate groups tend to be completely back-exchanged within the source, whilst the exchanged nucleobases continue to be labeled without detectable back-exchange. As a result, the change rates rely highly regarding the secondary construction (hydrogen bonding standing) of this oligonucleotides, but neither to their fee state nor from the presence of nonspecific adducts. We reveal that indigenous mass spectrometr
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