https://www.selleckchem.com/pr....oducts/eapb02303.htm
We found that on their own, sgRNA sequences do not produce a high degree of truncation events. However, we demonstrate that vector genome designs that carry dual sgRNA expression cassettes in tail-to-tail configurations lead to truncations. In addition, we revealed that heterogeneity in inverted terminal repeat sequences in the form of regional deletions inherent to certain AAV vector plasmids can be interrogated.The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for in vivo applications raises safety co
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