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To investigate the genetic diversity and phylogenetic relationship of isolates from snakes in Hunan Province. The partial mitochondrial NADH dehydrogenase subunit 4 ( ) and NADH dehydrogenase subunit 5 ( ) genes were amplified using a PCR assay in 7 isolates from snakes in Hunan Province and the amplification product was sequenced. The homology and genetic evolution were investigated using the software DNAMAN 7.0, MegAlign, DnaSP 5.0 and MEGA 5.0. The and gene sequences were approximately 578 bp and 484 bp in length in the 7 isolates fr
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