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Incubation of both cell lines with M22 (0.006-50 ng/mL) resulted in a dose-dependent increase in cAMP levels with linear ranges for the MC4 and WT cells of 0.8-12.5 and 0.2-3.125 ng/mL, respectively. Comparison of cAMP and luciferase levels in M22-treated MC4 and WT cells also showed a positive correlation (r = 0.88, p less then 0.001 and 0.75, p = 0.002). A positive correlation was also noted when using patient samples (r = 0.96, p less then 0.001) that were all TSH-R-Ab binding assay positive. Conclusion The novel, rapid, simple-to-pe
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