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© 2020 Wiley-VCH Verlag GmbH Co. KGaA, Weinheim.The baculovirus expression vector system is a very powerful tool to produce virus-like particles and gene-therapy vectors, but the removal of co-expressed baculovirus has been a major barrier for wider industrial use. We used chimeric HIV-1 gag influenza-HA VLPs produced in Tnms42 insect cells using the baculovirus insect cell expression vector system as model VLPs. A fast and simple purification method for these VLPs with direct capture and purification within one chromatography st
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