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The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R, respectively. Furthermore, the expression of TNF-α, 1L-6, 1L-1 and PAF were decreased after BNFY treatment. And the results of Western blot assay showed that BNFY downregulated TLR4, NF-κB, p-p38 MAPK expression and upregulated p-Akt expression. Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory respon
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