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Our results indicate that expression of mEGFP-tagged Nt-RanBP9 followed by thrombin cleavage of the tag was the most effective method to obtain the protein with 90% purity and good yields. We report and discuss the challenges and optimal methods to obtain the N-terminal region of RanBP9, a protein with functional implications in multiple biological processes and neurodegenerative diseases. OBJECTIVE To determine baseline knowledge of emergency medicine (EM) residents at 2 different residency training programs and assess if a 4-week
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