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The molecular components underlying antianoikis properties in cancer tumors cells continue to be mainly unclear. Here, we explain a protocol for planning of anoikis-resistant cultured nonmetastatic MCF-7 and metastatic MDA-MB-231 cell outlines. The anoikis-resistant cultures had been made by plating cells when you look at the poly-2-hydroxyethyl methacrylate coated plates and cultured for 24 h. The viability of cells when you look at the cultures ended up being determined utilizing trypan blue st
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