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When reseeded in normal serum-containing medium, the appearance amount of stemness biomarkers ended up being even higher in spheroid-derived ADSCs than monolayer culture. Significantly, spheroid ADSCs could effectively promote the M2 polarization of macrophages in both vitro plus in vivo. After transplantation into mouse, spheroid ADSCs improved the survival rate and considerably reduced serum levels of proinflammatory aspects IL-1β and TNF-α after LPS challenge. To sum up, we dev
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