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ctivity and to reduce manufacturing costs. Cellobiohydrolase from glycoside hydrolase family 7 is a major component of commercial enzymatic mixtures for lignocellulosic biomass degradation. For many years, Cel7A ( Cel7A) has served as a model to understand structure-function relationships of processive cellobiohydrolases. The architecture of Cel7A includes an -glycosylated catalytic domain, which is connected to a carbohydrate-binding module through a flexible, -glycosylated linker. Depending on the fungal expression host, glycosylatio
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