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With detection limits of 0.3555% and 1.500% for EGFR Del 19 and L858R mutations, respectively, the proposed TNT with PRF can effectively distinguish mutant cell lines and efficiently detect various lengths of genetic variations in clinical trials. © 2020 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.INTRODUCTION The analysis of plant and herbal samples is a challenging task for analytical chemists due to the complexity of the matrix combined with the low concentration of analytes. In recent years different liquid-phase microextraction (
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