https://www.selleckchem.com/
The Bacillus subtilis phage Phi29 has a linear double-stranded DNA with a terminal protein (TP) covalently linked to each 5' end (TP-DNA). Phi29 single-stranded DNA-binding protein (SS is encoded by the viral gene 5 and binds the ssDNA generated during the Phi29 genome replication, stimulating the DNA elongation rate. Here, we describe some protocols to evaluate the effect of Phi29 SSB mutants on the DNA elongation rate and their unwinding activity during replication by Phi29 DNA polymerase using as substrate TP-DNA and also singly primed M13 DNA.The sing
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