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This protocol calls for the following tips (1) determining the type of standard part which should be cloned, (2) designing primers for amplification, (3) performing PCR amplification, (4) cloning associated with the fragments utilizing Golden Gate cloning, and finally (5) sequencing associated with component. For large standard parts, it's better than very first clone subparts as advanced degree -1 constructs. These subparts tend to be sequenced individually and generally are then more assembled to help ma
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