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Background Accurate estimates of key population size are necessary to design programs and policies to prevent and reduce new HIV infections and to monitor the dynamics of the epidemic. The first Bio-behavioral Surveillance (BBS) surveys, with population size estimation activities, were conducted in Mozambique in 2010-2014. Methods We used multiple methods - sequential sampling, unique object, unique event and service multipliers - to estimate the numbers of men who have sex with men (MSM), female sex workers (FSW) and people who inject
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