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Pairwise comparison of ITS-2 sequences from this study and GenBank showed 98.5% homology for 80% of intra-species comparisons and less then 98.5% homology for 97% of inter-species comparisons, suggesting that some sequence data may have been entered into public repositories with incorrect species attribution based on morphological analysis. We propose that ITS-2 sequencing could be used as a universal tool for rumen fluke identification across host and parasite species from diverse technical and geographical origins and form the basis
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