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Somatic tissue sections, ovarian tissues, sperms, oocytes and embryos are potential materials for preservation by vitrification. As vitrification is economical and easily applied, it appears to the best option currently available for the preservation of wildlife female genetics in order to conserve species' biodiversity. We developed a hand-made silver container as a closed vitrification system (CVS) which avoid bacterial or viral contamination. The aim of this study was to evaluate the preservation outcomes by comparing silver CVS and s
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