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otrophs from forest to pasture and from pasture to secondary forest conversion. Studying the impact of land-use change on diazotrophs is important for a better understanding of the impact of deforestation on tropical forest ecosystem functioning and our results on the potential recovery of diazotrophs in secondary forest imply the possible restoration of ecosystem functions in secondary forests. Copyright © 2020 American Society for Microbiology.Random transposon mutagenesis is a powerful and unbiased genetic approach to answer funda
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