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In this research, according to a transcriptome assembly and annotation for the aquatic firefly Aquatica leii at the person and larval phases, 10 candidate reference genes (α-tubulin, β-tubulin, β-actin, EF1A, SDHA, UBQ, GST, GAPDH, RPS31, and RPL13A) were identified for analyses of appearance security. Quantitative real-time PCR analyses for each applicant reference genes in A. leii was carried out for four developmental stages, four adult tissue kinds, two adult sexes, as well a
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