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It was reported for the first time that the coverage density of TGA on functionalized monolithic column modified by GNPs was 168.41 nmol μL-1. Notably, the coverage density (2205.8 pmol μL-1) of the aptamer decorated on the hybrid monolithic column was significantly higher than most other similar materials in published works. After that, the aptamer functionalized hybrid monolithic column screened out was applied for the solid-phase microextraction of thrombin, which possessed excellent adsorption selectivity in interference experiment. Con
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