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The most prominently difference between the SERS spectrum of healthy brain tissue and that of gliomas at different grades is the reduction in quotient of two characteristic peaks at 653 and 724 cm-1. Furthermore, healthy brain tissue and Grade II gliomas as low grade gliomas as well as Grade III and Grade IV as high-grade gliomas can be clearly distinguished by three-dimensional PCA. Preliminary results indicate that the SERS spectra based on AgNPs@AgNR substrates can be applied for a rapid identification owing to its simple preparation o
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