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Anaerobic problems with the nutrient starvation resulted in mobile division obstruction, lowering of Chl a and remarkable alterations in pH, whereas a significant increase in the H2 production ended up being seen after 24 h. The greatest cumulative H2 productivity had been noticed in N-deficient medium (300 μL/L, time 9) followed by Mn-deficient medium (250 μL/L, day 7). The highest H2 production rate (3.37 μL/L/h) was accomplished by Mn-deficient medium after 24 h. In terms