https://interleukin-receptor.c....om/deep-multimodal-t
In Lactococcus lactis, a cell area screen precursor had been created, comprising DSHPQFC. The preceding Asp residue associated with serine in this series disfavors its dehydration by wild type NisB. The mobile area screen vector was co-expressed with a mutant NisB collection and NisTC. Later, mutant NisB-containing germs that display cyclized strep-ligands on the cell surface had been selected via panning rounds with streptavidin-coupled magnetic beads. In this manner, a NisB variant with a tailo
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