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Among all 240 patients with OS, 13 (5.4%) harbored a P/LP germline variant, of which 6 (46.2%) were confirmed to be de novo. Apart from , we did not observe any other recurrent de novo P/LP variants in the case-parent trios, suggesting that new mutations in other genes are not a frequent cause of pediatric OS. That nearly half of P/LP variants in our sample were de novo suggests universal screening for germline P/LP variants among pediatric patients with OS should be considered. Apart from TP53, we did not observe any other recurrent d
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