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Even though nebulised administration of amikacin can achieve high epithelial lining fluid concentrations, this has not translated into improved patient outcomes in clinical trials. One possible reason is that the cellular and chemical composition of the epithelial lining fluid may inhibit amikacin-mediated bacterial killing. The objective of this study was to identify whether the epithelial lining fluid components inhibit amikacin-mediated bacterial killing. Two amikacin-susceptible (minimum inhibitory concentrations of 2 and 8 mg/L) Ps
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