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In this study, we assembled the mitochondrial genome (mtDNA) regarding the Florida manatee from whole genome shotgun reads. As a result, we reveal that the presently annotated T. manatus mtDNA belongs to some other types, the Amazonian manatee (T. inunguis). The newly assembled Florida manatee mtDNA is 16,881 bp in total, with 13 protein-coding genes, two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and something non-coding control area (D-loop). Phylogenetic evaluation based on th
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