https://prostaglandinrecept.co....m/index.php/a-call-t
We now present a method according to genome-wide ligation of 3'-OH stops accompanied by sequencing (GLOE-Seq) and an associated computational pipeline designed for recording SSBs but flexible enough to be reproduced to virtually any lesion convertible into a free of charge 3'-OH terminus. We indicate its usefulness to mapping of Okazaki fragments without prior size choice and provide understanding of the general contributions of DNA ligase 1 and ligase 3 to Okazaki fragment maturation in hum
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