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Although cancer-associated fibroblasts (CAFs) are the most crucial stromal cells, characterizing their heterogeneity is far from complete. The authors report a novel subset of CAFs in oral squamous cell carcinoma (OSCC), which positively expressed CD68, the classic marker of macrophages. The spatial and temporal distribution of the CD68+ CAF subset of OSCC (n = 104) was determined by CD68/α-SMA immunohistochemistry of serial sections. The CD68+ α-SMA+ CAF subset was found to be elevated from dysplasia to OSCC. Moreover, although both the
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