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We call on the field of global-change biology to undertake these important challenges. Expected final online publication date for the Annual Review of Marine Science, Volume 14 is January 2022. Please see http//www.annualreviews.org/page/journal/pubdates for revised estimates.Genetic manipulations with a robust and predictable outcome are critical to investigate gene function, as well as for therapeutic genome engineering. For many years, knockdown approaches and reagents including RNA interference and antisense oligonucleotides dominat
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