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oleracea. Altogether, we provide essential tools for functional gene analysis in the context of C4/CAM photosynthesis, including an efficient RNA isolation method, preferred reference genes for RT-qPCR normalisation for a range of experimental conditions, and a protocol to produce P. oleracea stable transformants using A. tumefaciens.The present experiment was done on two different cultivars of a tropical legume, Cymopsis tetragonoloba L. Taub. (cluster bean) cvv. Pusa-Naubahar (PUSA-N) and Selection-151 (S-151). The experiment was conducted under a
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