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We isolated leukocytes through the mind renal of Astyanax lacustris-an important Neotropical seafood species for aquaculture and a possible model for Neotropical aquaculture research-using a Percoll centrifugation protocol. The separated leukocytes were incubated with lipopolysaccharide (LPS), while the expression of genetics IL-1β, IL-8, LysC, and LysG were measured. We evaluated the phagocytotic activity of leukocytes using Congo red-dyed yeast, a novel and cost-effective p
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