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26 pg mL-1. The designed strategy avoided complex synthesis of signal tags, and possessed excellent performance for analysis of practical samples, thus providing a new avenue for the development of signal amplification strategy and immunoassay methods.Prostate cancer associated 3 (PCA3) assay has been used to improve prostate cancer diagnosis and reduce unnecessary biopsies. In this work, we successfully developed a new PCA3 assay on an origami paper-based peptide nucleic acid device (oPAD). The PCA3 oPAD comprises an acrylic cassette an
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