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By adding chitosan to the medium to keep the fibers stable, 3D long-term in vitro cell co-culture has been carried out up to 21 days. This method is very simple and easy to operate, continuously produces spatially well-defined heterogeneous microfibers, has important applications for composite functional biomaterials, and shows great potential in organs-on-a-chip and biomimetic systems. © 2020 The Authors. Published by WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.Rhus coriaria L. (Anacardiaceae), sumac, is a common condiment, appetizer a
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