https://www.selleckchem.com/pr....oducts/picrotoxin.ht
Mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) recapitulate in vitro the epiblast first cell lineage decision, providing a powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.Advances in induced pluripotent stem cell (iPSC) technology provide a renewable source of cells for t
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