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The regulation of cell density is an important segment in microfluidic cell culture, particularly in the repeated assays. Traditionally, consistent cell density is difficult to achieve, owing to the inaccurate regulation of cell density with manual feedback. A novel cell culture method with automatic feedback is proposed for real-time regulation of cell density based on microfluidic chip in this paper. Here, an integrated microfluidic system combining cell culture, density detection, and control of proliferation rate was developed. Inter
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