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Hence, we further designed 3 linkers of different lengths to fuse LaG16 and GFP-enhancer collectively, while the GFP binding associated with the three constructs ended up being further tested by ITC. The construct with the (GGGGS)4 linker had the greatest affinity with a KD of 0.5 nM. The GFP-enhancer-(GGGGS)4-LaG16 chimeric nanobody ended up being additional covalently linked to NHS-activated agarose and then found in the purification of a G
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