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Wagyu crossbred steers (n = 167) were used to (1) compare the metabolome of individual animals at two distant time-points (days 196 and 432) in a feedlot (this corresponded to 272 and 36 days before slaughter); and (2) determine relationships between the metabolome and marbling, and the effect of days in the feedlot (time-points) on these relationships. 1H NMR spectroscopy followed by standard recoupling of variables analysis produced 290 features or 'peaks' from which 38 metabolites were identified. There was a positive correlation be
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