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As a key process contributing to N2O emissions, nitrification is regulated by soil microbes and mainly affected by soil pH, NH3 availability, temperature and O2 availability. Current knowledge gaps include how nitrification-related N2O is associated with soil microbes in different pH soils. In the current study, a microcosm incubation experiment was conducted with two contrasting soils of different pH (5.08, 8.3 under controlled conditions. The soils were amended with ammonium sulphate ((NH4)2SO4, 50 mg N kg-1) combined with or without
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