https://www.selleckchem.com/pr....oducts/plumbagin.htm
Raising read count from 2M to 10M drastically increased the accuracy of FF estimates. After adding more reads, we saw a further improvement in FF accuracy, reaching a plateau at 20M reads. Precision of SeqFF FF estimate is independent of FF level in the sample. SeqFF is a robust method for FF estimation for both genders and for any FF level in range 2-13%. Accuracy of FF estimates highly depends on the read count. We recommend using no less than 10M reads to achieve accurate FF estimates for NIPT analysis in clinical settings. SeqFF i
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