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This paper presents a combination of noninvasive techniques for the study of superimposed painting stages in The Pentecost, a Mexican Colonial panel painting attributed to Baltasar de Echave Orio (1558 - 1619). The application of reflected hyperspectral imaging (HSI) analysis for mapping the distribution of the pigments in the paint surface and the use of ultraviolet (UV) fluorescence photography and X-ray radiography as complementary imaging techniques provide new insights into the making process of the artwork, its manufacturing and con
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