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Further analysis demonstrated that deleting fungal MrGprk decreased the rates of appressorium formation and suppressed the transcription of several genes contributing to appressorial turgor pressure, cuticle penetration, and pH regulation. Additionally, the ΔMrGprk strain showed higher cyclic (cAMP) levels, suggesting that this GPCR is critical for cAMP signal transduction. In summary, MrGPRK was found to contribute to vegetative growth, conidial production, and full virulence of M. robertsii. These findings are conducive to a better un
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