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Silver nanoparticles (AgNPs) as antimicrobial agents have been extensively studied. It is generally assumed that their inhibitory activity heavily depends on their physicochemical features. Yet, other parameters may affect the AgNP traits and activity, such as culture medium composition, pH, and temperature, among others. In this work, we evaluated the effect of the culture medium physicochemical traits on both the stability and antibacterial activity of AgNPs. We found that culture media impact the physicochemical traits of AgNPs, such
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