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A rabbit polyclonal antiserum immunolabelled antigen of all three viruses within the lesions, whereas ISH specifically detected RNA of each individual virus. The distribution of viral RNA was comparable to that of viral antigens, but tended to be more widespread, especially in immature nervous tissue. Viral antigen and RNA were detected in skeletal muscle and heart of the rats infected with the KM-1 strain of Akabane virus but not with any of the other viruses. This study demonstrates the value of ISH detection of these viruses in a rat
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