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Taking advantage of the incessant cleavage activity of Cas13a and the high amplification efficiency of multiple LAMP reaction, as low as 1 fM target circRNA can be sensitively detected within 30 min. Due to the high specificity of Cas13a, the proposed assay has been successfully applied to the detection of circRNA in real biological samples without separation of corresponding linear RNAs. Moreover, the proposed assay has offered a versatile platform for the detection of all sequence-specific RNA targets, indicating that our CRISPR/Cas13a
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