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PK and hemoglobin (Hb) dimensions had been done on a Roche cobas c501 analyzer. After setting up a k-factor, reliability, linearity, imprecision, sensitiveness, and security were validated and also the reference period had been verified. Results The k-factor ended up being -9477. Accuracy ended up being assessed by strategy contrast (letter = 56). Linear regression yielded y = 1.0x - 0.57, and R2 of 0.93. Linearity ended up being determined by incorporating a higher sample with hemolyzing solution