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3-fold expansion for c-kit hematopoietic progenitor cells and 8.3-fold expansion for CD34 hematopoietic stem cells. In the controls, HC expansion was lower than in any cocultures, demonstrating the advantages of coculturing for HC expansion. Stromal cell lines are useful in expanding primitive HCs derived from FLCs in 3D cocultures. Culture density is a pivotal factor for the effective expansion of primitive HCs and this effect differs by culture condition. Stromal cell lines are useful in expanding primitive HCs derived from FLCs in 3D
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