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Sugarcane bagasse (SC was pretreated with hot water (HLW), ethanol (ETH), and sodium hydroxide (SH). The obtained residuals were hydrolyzed and applied as carbon sources for succinic acid (SA) fermentation, the residue digestibility and SA conversion rate of alkali-pretreated residual were superior to others. Considering the characteristics of alkali pretreatment, enzymatic hydrolysis and succinic acid fermentation, a novel in-situ semi-simultaneous saccharification and co-fermentation (SSSCF) procedure for SA production from SCB was
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