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Lactic acid bacteria had been separated from tea leaves cultured with de Man, Rogosa, and Sharpe (MRS) agar dishes, while the types were identified by homology of this 16 S rRNA gene and multiplex polymerase sequence reaction (PCR) of the recA gene to distinguish the Lactobacillus plantarum team. As a result, many different types had been separated from the natural tea leaves, and Lactobacillus pentosus had been separated most frequently after anaerobic fermentation. About the tea-leaf ele
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