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We established metastasis models for a cancerous colon to liver change by intrasplenic injection shRNA of NCKAP1-transfected HCT116 cells or by implanting cyst tissue created with all the cells on cecal pouch. In metastasis xenograft designs, tumor development and liver metastasis had been markedly paid off. Taken together, these information demonstrate that NCKAP1 is a novel gene regulating EMT that can subscribe to building a diagnostic marker when it comes t
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