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We resolve this substrate inhibition difficulty by increasing the inoculum density. Consequently, we used a fed-batch strategy by maintaining reduced HMF focus into the culture to maximise the last FDCA titre. Applying this approach, 545 mM of FDCA was accumulatively produced after 72 hs, that is the highest manufacturing rate per device size of cells to your most useful of our knowledge. © 2020 The Authors. Microbial Biotech
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