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01 ± 86.20 to 15.03 ± 1.5 mg/m3 , total nitrogen (TN) from 20.37 ± 4.64 to 12.10 ± 1.19 mg/L and total phosphorus (TP) from 2.05 ± 0.10 to 1.61 ± 0.24 mg/L. This article is protected by copyright. All rights reserved.We developed a new method for conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos via photochemically activated, caged guide RNAs. Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout the 5'-protospacer region with caged nucleobases during synthesis. Ca
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